Protein-sugar interactions: Environmental effect on the fluorescence of O-(4-methylumbelliferyl)-glycosides
We have investigated the effect of 12 solvents and several amino acids on the fluorescence of O-(4-methylumbelliferyl)-glycosides. We showed that: i) the fluorescence quenching is not related to the dielectric constant of the solvents: the fluorescence intensity was maximal in water (d=80) and in ac...
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Published in | Biochemical and biophysical research communications Vol. 110; no. 3; pp. 926 - 933 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
10.02.1983
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | We have investigated the effect of 12 solvents and several amino acids on the fluorescence of
O-(4-methylumbelliferyl)-glycosides. We showed that: i) the fluorescence quenching is not related to the dielectric constant of the solvents: the fluorescence intensity was maximal in water (d=80) and in acetic acid (d=6.2) and was at least ten times lower in acetone (d=21) and in dioxane (d=2.2); ii) the fluorescence of
O-(4-methylumbelliferyl)-
N-acetyl-β-glucosaminide is not quenched in the presence of various amino acids including arginine, asparagine, aspartate, histidine, leucine, phenylalanine and proline; iii) the fluorescence of
O-(4-methylumbelliferyl)-glycoside is quenched by sulfur, phenol and indole amino acids or derivatives containing sulfur, phenol or indole groups. The changes in fluorescence intensities of
O-(4-methylumbelliferyl)-glycosides upon binding to concanavalin A, wheat germ agglutinin and lysozyme are discussed with regard to the amino acid content of their binding sites. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/0006-291X(83)91051-3 |