Characteristic MALDI-MS lipid profiles of Gir, Holstein and crossbred (Gir x Holstein) oocytes recovered by ovum pick-up

• Published in: Livestock science Vol. 243; p. 104380
• Main Authors: Marsico, Thamiris Vieira, Sales, José Nélio de Sousa, Ferreira, Christina Ramires, Sudano, Mateus José, Viana, João Henrique Moreira, Camargo, Luiz Sérgio de Almeida, Eberlin, Marcos Nogueira, Seneda, Marcelo Marcondes, Baruselli, Pietro Sampaio
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2021
• Subjects: Cattle; Crossbreed; Dairy breeds; Lipid profile; Metabolism; Oocyte; Lipid profile; Dairy breeds; Metabolism; Crossbreed; Cattle; Oocyte
• ISSN: 1871-1413; 1878-0490
• DOI: 10.1016/j.livsci.2020.104380

•A wide-range MALDI-MS lipid characterization of dairy breeds oocytes were provided.•The lipid profiles of the oocytes could be discriminated between the pure breeds.•Gir and crossbred oocytes were predominantly represented by phospholipids.•Holstein oocytes were predominantly enriched in triacylglycerol. The differential expression of many productive and reproductive traits has already been associated with the genotype effect observed between Bos taurus indicus (Bos indicus) and Bos taurus taurus (Bos taurus) breeds. Lipids have a crucial role during oocyte development. The aim of this work was to characterize the lipid profiles of oocytes recovered from dairy breeds: Gir (Bos indicus), Holstein (Bos taurus) and their crossbred (Gir x Holstein). Cumulus-oocyte complexes were collected by ovum pick-up from Gir (n=21), Holstein (n=36), and crossbreed (n=33) cows and submitted to MALDI-MS lipid fingerprint analysis. Multi- and univariate analyses were used to characterize lipid profiles of oocytes derived from each breed. Third lipid profiles (Gir n=7; Holstein n=12; and crossbred n=11) were evaluated and allowed characterization of oocyte's lipids among breeds. The lipid profiles from Gir and Holstein were observed to be the most distinct, whereas Gir and crossbred oocytes presented greater similarities. Overall, a wide-range oocyte lipid profile characterization from distinctive bovine dairy breeds recovered by OPU were provided. We have identified two distinguishable lipid profile patterns among groups and revealed that phospholipids and triacylglycerol were the predominantly lipid assigned in Gir (Bos indicus) and Holstein (Bos taurus) oocytes, respectively.