Preparation and properties of a hemoglobin containing heme only in gamma subunits

A semihemoglobin containing prosthetic groups only in the γ-subunits (two hemes per tetramer) has been prepared by mixing together apo-α-subunits from hemoglobin A and native, heme-containing γ-subunits from hemoglobin F. The semihemoglobin has optical properties similar to those of hemoglobin F. Th...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 56; no. 2; pp. 444 - 450
Main Authors Gondko, Roman, Obrebska, Maria J., Waterman, Michael R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 23.01.1974
Subjects
Apoproteins
Carbon Monoxide
Carboxyhemoglobin
Chromatography, Ion Exchange
Electrophoresis, Starch Gel
Female
Fetal Hemoglobin
Hemoglobins - isolation & purification
Humans
Mercuribenzoates
Oxygen
Oxyhemoglobins
Peptides - analysis
Pregnancy
Protein Binding
Spectrophotometry
Spectrophotometry, Ultraviolet
Umbilical Cord
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Summary:A semihemoglobin containing prosthetic groups only in the γ-subunits (two hemes per tetramer) has been prepared by mixing together apo-α-subunits from hemoglobin A and native, heme-containing γ-subunits from hemoglobin F. The semihemoglobin has optical properties similar to those of hemoglobin F. The oxygen affinity of the semihemoglobin is lower than that of isolated γ-subunits but not as low as that of hemoglobin F, and the Hill coefficient of the semihemoglobin is near one. This semihemoglobin lends further support to the non-equivalence of the subunits in the hemoglobin tetramer.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(74)90862-6