Type-A retrovirus particles in heterohybridomas: Precaution for human monoclonal antibody purification

Fine structural features of the murine myeloma MHFP-1 and two heterohybridomas secreting human IgM monoclonal antibody were examined. Intracisternal type-A retrovirus particles were found in both MHFP-1 and the heterohybridomas constructed by fusing MHFP-1 and human peripheral blood lymphocytes. The...

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Bibliographic Details
Published inJournal of ultrastructure research Vol. 92; no. 1; pp. 80 - 85
Main Authors Herr, John C., Spell, Donald R.
Format Journal Article
LanguageEnglish
Published United States Elsevier B.V 01.07.1985
Subjects
Animals
Antibodies, Monoclonal - isolation & purification
Cell Line
Hybridomas - immunology
Hybridomas - microbiology
Hybridomas - ultrastructure
Mice
Microscopy, Electron
Plasmacytoma - microbiology
Plasmacytoma - ultrastructure
Retroviridae - ultrastructure
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Summary:Fine structural features of the murine myeloma MHFP-1 and two heterohybridomas secreting human IgM monoclonal antibody were examined. Intracisternal type-A retrovirus particles were found in both MHFP-1 and the heterohybridomas constructed by fusing MHFP-1 and human peripheral blood lymphocytes. The implications of this finding for the purification of human monoclonal antibody for therapeutic applications is discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-5320
1878-2345
DOI:10.1016/0889-1605(85)90130-2