Effect of Five Commercial Probiotic Formulations on Candida Albicans Growth: In Vitro Study

• Published in: The Journal of clinical pediatric dentistry Vol. 44; no. 5; pp. 315 - 322
• Main Authors: Hernández-Bautista, Liz Mariana, Márquez-Preciado, Raúl, Ortiz-Magdaleno, Marine, Pozos-Guillén, Amaury, Aranda-Romo, Saray, Sánchez-Vargas, Luis Octavio
• Format: Journal Article
• Language: English
• Published: Birmingham Journal of Clinical Pediatric Dentistry 01.09.2020
• Subjects: Acids; Bacteria; Dentistry; Laboratories; Microorganisms; Pediatrics; Probiotics
• ISSN: 1053-4628; 1557-5268
• DOI: 10.17796/1053-4625-44.5.5

Purpose: The objective was to evaluate the antagonistic effect of Lactobacillus and Bifidobacterium recovered from five commercial probiotics on the growth of C. albicans. Study design: The Lactobacillus and Bifidobacterium strains of five commercial probiotics were recovered and grown: Probio Hp+®, ProBiseis®, Lactipan®, Liolactil®, and Lacteol Fort®; 50 mg of each was hydrated and grown in Lactobacilli MRS (De Man, Rogosa and Sharpe) broth and incubated at 37°C with stirring (120 RPM) for 24 hours. Serial dilutions of 10−1 to 10−7 were made and viability was verified and quantified. For the antagonism tests, a suspension/inoculum of Lactobacillus strains recovered from each commercial preparation (4–30 × 109) and C. albicans ATCC 90028 (1.5–8 × 108) was prepared in MRS broth and incubated for 48 hours at 36°C, then plated on Dextrose Sabouraud Agar with Chloramphenicol and Rogosa Agar and the colony-forming units (CFU) were quantified. Additionally, viability was evaluated using the LIVE/DEAD® Yeast and Bacterial Viability kit. Results: The probiotic that produced the highest acidity of the medium was Lactipan®, followed by Probiseis® and Liolactil®, while Probio Hp+® showed the least change. Probiseis® was determined to have the highest growth of probiotic bacteria and the highest inhibition on C. albicans, followed by Lactipan®; Liolactil® and ProbioHp+® showed the least effect. In fluorescence tests, ProBiseis® showed the best effect, followed by Liolactil® and Lactipan®; Probio Hp+® had less of an effect. Conclusions: Two commercial products (ProBiseis and Lactipan) whose formulations have L. acidophilus, L. casei, L. rhamnosus, L. plantarum, B. infantis, and S. thermophilus have a greater inhibitory effect on C. albicans ATCC 90028