Dual-functionalized titanium for enhancing osteogenic and antibacterial properties

• Published in: Colloid and interface science communications Vol. 44; p. 100481
• Main Authors: Tang, Qiang, Zhang, Xiaojing, Shen, Ke, Zhu, Zhaojing, Hou, Yanhua, Lai, Min
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2021
• Subjects: Antibacterial property; Gentamicin sulfate; Naringin; Osteoblast; TiO2 nanotubes; Osteoblast; TiO2 nanotubes; Naringin; Gentamicin sulfate; Antibacterial property
• ISSN: 2215-0382; 2215-0382
• DOI: 10.1016/j.colcom.2021.100481

Bacterial infection and poor osseointegration of titanium implants are two of the main issues hindering their use in clinical applications. In order to solve these problems simultaneously, TiO2 nanotubes were fabricated, loaded with naringin (NA) and covered by gentamicin sulfate (GS)-inserted chitosan/gelatin bilayers through layer by layer technique. The successful system of the bifunctional titanium substrate was confirmed via scanning electron microscopy, atomic force microscope, water contact angle measurement, X-ray photoelectron spectroscopy and in vitro release of NA and GS. The multilayer-coated NA-loaded TiO2 nanotubes exhibited good biocompatibility as shown by cell morphology, cell viability assay, alkaline phosphatase activity, mineralization and qRT-PCR analysis of the osteogenesis related genes. Antibacterial experiments examining S. aureus and E. coli in vitro demonstrated that the bifunctional TiO2 nanotubes had antibacterial capabilities. Ihis approach provides a new and feasible strategy for improving the properties of titanium implants to promote osteogenic and antibacterial activity. [Display omitted] •The naringin loaded-TiO2 nanotubes were covered by gentamicin sulfate-inserted multilayered films through LBL technique.•The bifunctional titanium substrates displayed controlled release of naringin and gentamicin sulfate.•The bifunctional titanium substrates had great potential for promoting osteoblast differentiation.•The bifunctional titanium substrates effectively displayed antibacterial activity.