Studies on insect ( Periplaneta americana L.) phosphofructokinase

1. 1. The presence of phosphofructokinase activity in different tissues from the cockroach ( Periplaneta americana) has been investigated. 2. 2. The coxal muscles enzyme was studied at different F6P concentrations, and an apparent K m of 6·9 × 10 −5 M was calculated. 3. 3. The enzyme shows an optimu...

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Bibliographic Details
Published inComparative biochemistry and physiology Vol. 26; no. 3; pp. 979 - 984
Main Authors Grasso, A., Migliori Natalizi, G.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.01.1968
Subjects
G6P
NAD
F6P
FDP
5′-AMP
Pi
ATP
3′5′-AMP (cyclic)
PFKase
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Summary:1. 1. The presence of phosphofructokinase activity in different tissues from the cockroach ( Periplaneta americana) has been investigated. 2. 2. The coxal muscles enzyme was studied at different F6P concentrations, and an apparent K m of 6·9 × 10 −5 M was calculated. 3. 3. The enzyme shows an optimum activity at pH 7·5. 4. 4. ATP strongly inhibits enzyme activity. 5. 5. 5′-AMP, Pi, NH 4 + caused a marked increase in enzyme activity, while the enzyme is inhibited by ATP. 6. 6. 3′5′-AMP (cyclic) at 0·22 mM ATP partially increases enzyme activity. 7. 7. The above reported data strongly suggest that the enzyme is a site of hormonal control in glycolysis.
ISSN:0010-406X
DOI:10.1016/0010-406X(68)90018-2