New lanostane-type triterpenoids, inonotsutriols D, and E, from Inonotus obliquus

• Published in: Phytochemistry letters Vol. 4; no. 3; pp. 328 - 332
• Main Authors: Tanaka, Reiko, Toyoshima, Misaki, Yamada, Takeshi
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.09.2011
• Subjects: Hymenochaetaceae; Inonotsutriol D; Inonotsutriol E; Inonotus obliquus; Leukemia; Inonotsutriol D; Inonotus obliquus; Inonotsutriol E; Hymenochaetaceae; Leukemia
• ISSN: 1874-3900; 1876-7486
• DOI: 10.1016/j.phytol.2011.07.001

New triterpenoids named inonotsutriols D ( 1) and E ( 2) were isolated from I. obliquus. Their structures were determined as lanost-8-ene-3β,22 R,24 R-triol ( 1) and lanost-8-ene-3β,22 R,24 S-triol ( 2). Compound 1 exhibited cytotoxic activity against four cancer cell lines (P388, L1210, HL-60, and KB). [Display omitted] ► New triterpenoids, inonotsutriols D ( 1) and E ( 2) were isolated from I. obliquus. ► The structure of 1 was determined as lanost-8-ene-3β,22 R,24 R-triol. ► The structure of 2 was determined as lanost-8-ene-3β,22 R,24 S-triol. ► Cytotoxicities of compounds 1, 3– 8 were examined using P388, L1210, HL-60, and KB cell lines. ► Compound 1 exhibited cytotoxic activity against all four cancer cell lines. Two new lanostane-type triterpenoids, inonotsutriols D ( 1) and E ( 2), were isolated from the sclerotia of Inonotus obliquus (Pers.: Fr.) Pil. (Japanese name: kabanoanatake; Russian name: chaga). Their structures were determined to be lanost-8-ene-3β,22 R,24 R-triol ( 1) and lanost-8-ene-3β,22 R,24 S-triol ( 2) on the basis of spectral data, including 2D NMR analysis. In addition, major compounds, inotodiol ( 3), trametenolic acid ( 4), 3β-hydroxylanosta-8,24-dien-21-al ( 5), 21-hydroxylanosterol ( 6), inonotsuoxide A ( 7) and inonotsuoxide B ( 8) were identified, and all compounds, except 2, were evaluated for their cancer cell growth inhibitory activity against P388, HL-60, L1210 and KB cell lines.