Interaction of bovine serum albumin with Acridine Orange (C.I. Basic Orange 14) and its sonodynamic damage under ultrasonic irradiation

The interaction of acridine orange (C.I. Basic Orange 14) with bovine serum albumin and the subsequent sonodynamic damage to bovine serum albumin imparted by ultrasonic irradiation were studied. The quenching constant, binding constant and the binding site number were measured using fluorescence que...

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Bibliographic Details
Published inDyes and pigments Vol. 80; no. 2; pp. 271 - 278
Main Authors Wang, Jun, Zhang, Yuan-Yuan, Guo, Ying, Zhang, Lei, Xu, Rui, Xing, Zhi-Qiang, Wang, Shi-Xian, Zhang, Xiang-Dong
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.02.2009
Subjects
Acridine orange dye
Bovine serum albumin
Damage
Interaction
Sonodynamics
Ultrasonic irradiation
Bovine serum albumin
Acridine orange dye
Sonodynamics
Damage
Ultrasonic irradiation
Interaction
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Summary:The interaction of acridine orange (C.I. Basic Orange 14) with bovine serum albumin and the subsequent sonodynamic damage to bovine serum albumin imparted by ultrasonic irradiation were studied. The quenching constant, binding constant and the binding site number were measured using fluorescence quenching. The distance of binding between the dye and the serum albumin was obtained according to Föster's nonradiative energy transfer theory. The effects of ultrasonic irradiation time, dye concentration, pH and ionic strength on bovine serum albumin were determined using UV–vis and fluorescence spectra; the extent of damage was enhanced by a increases in irradiation time, dye concentration and ionic strength, but was reduced by an increase in pH.
ISSN:0143-7208
1873-3743
DOI:10.1016/j.dyepig.2008.07.013