Simple fluorimetric determination of selenium in food and biological materials. A validation study

A method for the determination of selenium in food and biological materials has been developed. The method involves sample decomposition in a closed system at 150 degrees C with nitric acid, perchloric acid treatment of the digest, reduction of selenium to the tetravalent state with hydrochloric aci...

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Bibliographic Details
Published inZeitschrift für Lebensmittel-Untersuchung und -Forschung Vol. 181; no. 3; pp. 189 - 193
Main Authors Vaessen, H.A.M.G, Van Ooik, A, Zuydendorp, J
Format Journal Article
LanguageEnglish
Published Germany 01.09.1985
Subjects
Animals
bioassays
fluorimetry
food analysis
Food Analysis - methods
Indicators and Reagents
selenium
Selenium - analysis
Spectrometry, Fluorescence
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Summary:A method for the determination of selenium in food and biological materials has been developed. The method involves sample decomposition in a closed system at 150 degrees C with nitric acid, perchloric acid treatment of the digest, reduction of selenium to the tetravalent state with hydrochloric acid followed by Se-2,3-diaminonaphthalene (DAN) complex formation and extraction in a single-step operation. Performance characteristics of the method (accuracy and repeatability) were extensively tested for eleven reference materials and ten practice samples covering both a wide variety of matrices and a wide range of selenium content. Almost all results for reference materials were within the 95% confidence range of the certified value or the best estimate thereof. The coefficient of variation for all samples ranged from 2.3 to 10.1%, average value 4.7%. Average recovery for spiking experiments-levels 0.060, 0.075 and 0.150 micrograms/g was 102%; range 84-114%.
ISSN:0044-3026
1438-2385
DOI:10.1007/BF02425575