Determination of low-activity hydrolases using ESIPT fluorescent indicators on silver surfaces

The article presents an approach for determination of low activity hydrolases down to the nanoscale amount. It consists in a specific design of fluorescent indicator using Excited-State Intramolecular Proton-Transfer (ESIPT) phenomenon and enhancement of its fluorescence on the metal surface (Metal...

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Bibliographic Details
Published inDyes and pigments Vol. 149; pp. 224 - 228
Main Authors Serdiuk, Illia E., Reszka, Milena, Synak, Anna, Liberek, Beata, Bojarski, Piotr
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.02.2018
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Summary:The article presents an approach for determination of low activity hydrolases down to the nanoscale amount. It consists in a specific design of fluorescent indicator using Excited-State Intramolecular Proton-Transfer (ESIPT) phenomenon and enhancement of its fluorescence on the metal surface (Metal Enhanced Fluorescence – MEF). The developed approach is tested with β-glucosidase using novel ESIPT fluorescent indicator from the flavonol family. [Display omitted]
ISSN:0143-7208
1873-3743
DOI:10.1016/j.dyepig.2017.09.067