High resolution melting (HRM) of forensically informative SNPs

The SNaPshot® assay is commonly used for forensic SNP analysis. However, it is a multi-step process with potential post-PCR contamination risk. The single tube high resolution melting (HRM) temperature real-time PCR method is an alternative, eliminating the post-PCR tube transfer of SNaPshot®. Eight...

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Bibliographic Details
Published inForensic science international. Genetics supplement series Vol. 4; no. 1; pp. e376 - e377
Main Authors Mehta, Bhavik, Daniel, Runa, McNevin, Dennis
Format Journal Article
LanguageEnglish
Published Elsevier Ireland Ltd 2013
Subjects
High resolution melt
HRM
IrisPlex
SNaPshot
SNP genotyping
SNPs
SNPs
High resolution melt
IrisPlex
HRM
SNP genotyping
SNaPshot
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Summary:The SNaPshot® assay is commonly used for forensic SNP analysis. However, it is a multi-step process with potential post-PCR contamination risk. The single tube high resolution melting (HRM) temperature real-time PCR method is an alternative, eliminating the post-PCR tube transfer of SNaPshot®. Eight individual DNA samples were genotyped at the six IrisPlex SNP loci using both the IrisPlex published primer set and a set of custom designed HRM primers. The performance of MeltDoctor™ (Life Technologies®) and SensiFast™ (Bioline®) HRM mastermixes was examined on the ViiA™ 7 Real Time PCR platform for 10ng and 1ng DNA template amounts. The resultant genotypes were compared with those derived from SNaPshot®. This preliminary study demonstrates HRM potentially offers a fast and flexible alternative to SNaPshot® for small numbers of SNP loci without the associated contamination risk from post-PCR processes.
ISSN:1875-1768
1875-175X
DOI:10.1016/j.fsigss.2013.10.191