Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1

•Self-assembled monolayer on biotinylated oligonucleotide gold.•Label-free impedimetric detection of DNA hybridization.•Impedimetric detection for HIV-1 proviral DNA. This study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (S...

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Published inMicrochemical journal Vol. 153; p. 104462
Main Authors Tamayo, Abel I. Balbin, Rizo, Laura S. López, de Armas, Madelin Blanco, Ferreira, Antonio Aparecido Pupim, Manzani, Danilo, Yamanaka, Hideko, Guas, Ana Margarita Esteva
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.03.2020
Subjects
DNA biosensors
Genosensor
HIV-1
Hybridization
Impedimetric
Proviral DNA
HIV-1
DNA biosensors
Genosensor
Proviral DNA
Hybridization
Impedimetric
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Summary:•Self-assembled monolayer on biotinylated oligonucleotide gold.•Label-free impedimetric detection of DNA hybridization.•Impedimetric detection for HIV-1 proviral DNA. This study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (SAM). The sensor was composed of a gold rod with a SAM of biotin-modified 19-base oligonucleotide probe (5´-AAA GAT TAA TCC TGG G -3´,) for HIV-1. The electrode modification was characterized by Surface-Enhancement Raman Spectroscopy (SERS), cyclic voltammetry and the hybridization with the complementary oligonucleotide was monitored by impedimetric technique. SERS was used to confirm the linking of Biotin-DNA on the surface of the Au electrode. The coverage was 89% of the surface and a concentration of complementary oligonucleotides of 2.5 10−12 molL−1 could be detected. [Display omitted]
ISSN:0026-265X
1095-9149
DOI:10.1016/j.microc.2019.104462