Improvement of the operational stability of the Novozym 435® biocatalyst in the oxidation of 2,5-furandicarboxaldehyde to 2,5-furandicarboxylic acid in a continuous packed-bed microbioreactor

• Published in: Tetrahedron letters Vol. 121; p. 154475
• Main Authors: Gómez-Meyer, Daniel, Guajardo, Nadia, Lienqueo, María Elena
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 17.05.2023
• Subjects: 2,5-Furandicarboxaldehyde (DFF); 2,5-Furandicarboxylic acid (FDCA); Chemoenzymatic oxidation; Continuous oxidation; Microbioreactor; Microbioreactor; Continuous oxidation; 2,5-Furandicarboxylic acid (FDCA); Chemoenzymatic oxidation; 2,5-Furandicarboxaldehyde (DFF)
• ISSN: 0040-4039; 1873-3581
• DOI: 10.1016/j.tetlet.2023.154475

[Display omitted] The present work reports the evaluation of the yield, productivity and operational stability of the chemoenzymatic oxidation of 2,5-furandicarboxaldehyde (DFF) to 2,5-furan dicarboxylic acid (FDCA) catalyzed by immobilized lipase B from Candida antarctica (Novozym® 435) in a continuous packed bed microbioreactor. The highest yields and productivities were achieved using a feed for the reaction medium at 36 µL/min flow. The yield and specific productivity in continuous packed bed microbioreactor were approximately 20% and 1000 times higher than that obtained by operating in millibioreactors. The operational stability of the biocatalyst in the microbioreactor was higher than that achieved in a millibioreactor in continuous operation for 24 h. Finally, the packed bed microbioreactor is an interesting and straightforward strategy to increase the stability of the biocatalyst in continuous mode.