Towards hyperpolarized 13C-succinate imaging of brain cancer

We describe a novel 13C enriched precursor molecule, sodium 1- 13C acetylenedicarboxylate, which after hydrogenation by PASADENA (Parahydrogen and Synthesis Allows Dramatically Enhanced Nuclear Alignment) under controlled experimental conditions, becomes hyperpolarized 13C sodium succinate. Fast in...

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Bibliographic Details
Published inJournal of magnetic resonance (1997) Vol. 186; no. 1; pp. 150 - 155
Main Authors Bhattacharya, Pratip, Chekmenev, Eduard Y., Perman, William H., Harris, Kent C., Lin, Alexander P., Norton, Valerie A., Tan, Chou T., Ross, Brian D., Weitekamp, Daniel P.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.05.2007
Subjects
13C
9L tumor
Acetylenedicarboxylate
Ex vivo spectroscopy
Glutamine
Hyperpolarization
In vivo imaging
PASADENA
Succinate
13C
9L tumor
Succinate
Acetylenedicarboxylate
PASADENA
Hyperpolarization
Glutamine
In vivo imaging
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Summary:We describe a novel 13C enriched precursor molecule, sodium 1- 13C acetylenedicarboxylate, which after hydrogenation by PASADENA (Parahydrogen and Synthesis Allows Dramatically Enhanced Nuclear Alignment) under controlled experimental conditions, becomes hyperpolarized 13C sodium succinate. Fast in vivo 3D FIESTA MR imaging demonstrated that, following carotid arterial injection, the hyperpolarized 13C-succinate appeared in the head and cerebral circulation of normal and tumor-bearing rats. At this time, no in vivo hyperpolarized signal has been localized to normal brain or brain tumor. On the other hand, ex vivo samples of brain harvested from rats bearing a 9L brain tumor, 1 h or more following in vivo carotid injection of hyperpolarized 13C sodium succinate, contained significant concentrations of the injected substrate, 13C sodium succinate, together with 13C maleate and succinate metabolites 1- 13C-glutamate, 5- 13C-glutamate, 1- 13C-glutamine and 5- 13C-glutamine. The 13C substrates and products were below the limits of NMR detection in ex vivo samples of normal brain consistent with an intact blood–brain barrier. These ex vivo results indicate that hyperpolarized 13C sodium succinate may become a useful tool for rapid in vivo identification of brain tumors, providing novel biomarkers in 13C MR spectral-spatial images.
ISSN:1090-7807
1096-0856
DOI:10.1016/j.jmr.2007.01.017