Expression of Ion Channels in Perivascular Stem Cells derived from Human Umbilical Cords
Potassium channels, the largest group of pore proteins, selectively regulate the flow of potassium (K ) ions across cell membranes. The activity and expression of K channels are critical for the maintenance of normal functions in vessels and neurons, and for the regulation of cell differentiation an...
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Published in | Balsaeng'gwa saengsig Vol. 21; no. 1; pp. 11 - 18 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Korea (South)
한국발생생물학회
01.03.2017
The Korean Society of Developmental Biology |
Subjects | |
Online Access | Get full text |
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Summary: | Potassium channels, the largest group of pore proteins, selectively regulate the flow of potassium (K
) ions across cell membranes. The activity and expression of K
channels are critical for the maintenance of normal functions in vessels and neurons, and for the regulation of cell differentiation and maturation. However, their role and expression in stem cells have been poorly understood. In this study, we isolated perivascular stem cells (PVCs) from human umbilical cords and investigated the expression patterns of big-conductance Ca
-activated K
(BK
) and voltage-dependent K
(K
) channels using the reverse transcription polymerase chain reaction. We also examined the effect of high glucose (HG, 25 mM) on expression levels of BK
and K
channels in PVCs. K
1.1, K
β
, K
1.3, K
3.2, and K
6.1 were detected in undifferentiated PVCs. In addition, HG treatment increased the amounts of BK
β
, BK
β
, K
1.3, K
1.6, and K
6.1 transcripts. These results suggested that ion channels may have important functions in the growth and differentiation of PVCs, which could be influenced by HG exposure. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 G704-001746.2017.21.1.003 |
ISSN: | 2465-9525 2465-9541 |
DOI: | 10.12717/DR.2017.21.1.011 |