Isolation and characterization of disc shaped phycobilisomes from the red alga Rhodella violacea

Disc-shaped phycobilisomes were purified from Triton X100 treated cell homogenates of the unicellular marine red alga, Rhodella violacea. Their absorption spectrum had principal maxima at 544 and 568 nm (B-phycoerythrin), 624 nm (C-phycocyanin) and a distinct shoulder at 652 nm (allophycocyanin). In...

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Bibliographic Details
Published inArchives of microbiology Vol. 112; no. 1; pp. 61 - 67
Main Authors Koller, K.P, Wehrmeyer, W, Schneider, H
Format Journal Article
LanguageEnglish
Published Germany 01.01.1977
Subjects
Cell Fractionation
Cytoplasmic Granules - ultrastructure
Microscopy, Electron
Phycobilisomes
Phycocyanin - analysis
Phycoerythrin - analysis
Pigments, Biological - analysis
plant biochemistry
plant physiology
Rhodophyta - analysis
Rhodophyta - ultrastructure
Spectrophotometry
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Summary:Disc-shaped phycobilisomes were purified from Triton X100 treated cell homogenates of the unicellular marine red alga, Rhodella violacea. Their absorption spectrum had principal maxima at 544 and 568 nm (B-phycoerythrin), 624 nm (C-phycocyanin) and a distinct shoulder at 652 nm (allophycocyanin). Intermolecular energy transfer within the phycobilisomes was clearly demonstrated by fluorescence data. Excited at 546 nm intact phycobilisomes showed a main fluorescence emission maximum at 665 nm, a minor one at 577 nm and a shoulder at 730 nm. Dissociated phycobilisomes revealed a composition of 58% B-phycoerythrin, 25% C-phycocyanin and 17% allophycocyanin under the cultural conditions used. Analytical methods resolved no other components than phycobiliproteins. In addition to the defined C-phycocyanin and two isoproteins of B-phycoerythrin a stable heterogeneous aggregate of B-phycoerythrin/C-phycocyanin was separated in considerable amounts. In the electron microscope negatively stained phycobilisomes appeared as elliptical aggregates having dimensions slightly above the values found in ultrathin sections and a detailed subunit structure. All observations and data suggest a new rhodophytan phycobilisome type in Rhodella violacea.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00446655