Neiella holothuriorum sp. nov., isolated from the gut of a sea cucumber Apostichopus japonicus

• Published in: Antonie van Leeuwenhoek Vol. 115; no. 4; pp. 497 - 503
• Main Authors: Bai, Xin-Feng, Lv, Xue-Lian, Liu, Xue, Cui, Ting-Ting, Zhang, Mian-Song, Ding, Ning, Liu, Chang-Heng, Jia, Ai-Rong
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.04.2022; Springer Nature B.V
• Subjects: Apostichopus japonicus; Biomedical and Life Sciences; Deoxyribonucleic acid; DNA; Fatty acids; Flagella; Gene sequencing; Genomes; Genomics; Group theory; Hybridization; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Nucleotide sequence; Nucleotides; Original Paper; Phosphatidylethanolamine; Phosphatidylglycerol; Phylogeny; Plant Sciences; Quinones; rRNA 16S; Sodium chloride; Soil Science & Conservation; Intestinal content; Sea cucumber; Bacterial taxonomy; Neiella
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-022-01713-z

A Gram-stain negative, aerobic, rod-shaped bacterium, designated 126 T , was isolated from the intestinal content of a sea cucumber, Apostichopus japonicus , in China. Strain 126 T was found to grow optimally at 25–28 °C and pH 7.5–8.0 in marine 2216 E medium, with tolerance of 1–7% (w/v) NaCl. Strain 126 T is motile by means of one to several polar flagella. The dominant fatty acids of strain 126 T were identified as C 16:1 ω7 c /C 16:1 ω6 c (29.5%), C 18:1 ω7 c /C 18:1 ω6 c (19.8%) and C 16:0 (16.7%). The respiratory quinone was found to be Q-8. The polar lipid profile was found to be mainly composed of phosphatidylglycerol and phosphatidylethanolamine. The total length of the draft genome is approximately 4.2 × 10 6  bp, encoding 3655 genes and 3576 coding sequences. The G + C content of the genomic DNA is 48.0%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 126 T belongs to the genus Neiella and is closely related to Neiella marina J221 T (96.5%). Genomic comparisons of 126 T to N. marina J221 T revealed that they had similar genome size, G + C content and complement of clusters of orthologous groups. However, average nucleotide identity and digital DNA–DNA hybridization values between strains126 T and N. marina J221 T was 75.5% and 19.7%, which could distinguish the strains. On the basis of these phenotypic and genotypic data, strain 126 T is concluded to represent a novel species, for which the name Neiella holothuriorum sp. nov. is proposed. The type strain is 126 T (= GDMCC 1.2530 T  = KCTC 82829 T ).