Integrated analysis of miRNA and mRNA expression profiles in diabetic mouse kidney treated to Korean Red Ginseng

• Published in: Molecular & cellular toxicology Vol. 18; no. 4; pp. 637 - 646
• Main Authors: Park, Hye Rim, Lee, Seung Eun, Yi, Yoojung, Moon, Sangji, Yoon, Hyunkyung, Kang, Chae Won, Kim, Jaetaek, Park, Yong Seek
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.10.2022; Springer Nature B.V; 대한독성 유전단백체 학회
• Subjects: Biomedical and Life Sciences; Cell Biology; Diabetes; Diabetes mellitus; Gene expression; Ginseng; Herbal medicine; Kidneys; Life Sciences; miRNA; Nephropathy; Original Article; Pharmacology/Toxicology; Public health; Streptozocin; Transcriptomes; 생물학; Korean Red Ginseng; Diabetic nephropathy; miRNA
• ISSN: 1738-642X; 2092-8467
• DOI: 10.1007/s13273-022-00272-8

Background Diabetes mellitus (DM) and its complications, including diabetic nephropathy, are rapidly increasing public health concerns. Korean Red Ginseng (KRG) is a popular herbal medicine that has many biological effects. The current literature demonstrates that KRG induced changes to the transcriptome in diabetic nephropathy mouse model are limited. Thus, we designed experiments to confirm whether changes of genes and miRNAs expression patterns in the renal cells of mice with DM, and then whether these changes are reversed by KRG. Objective This study aims to whether miRNAs and mRNAs expression and their relationships were contributed in the renal tissues of mice with streptozotocin (STZ)-induced diabetes. Results We determined that the expression levels of 46 miRNAs and 827 mRNAs expression were changed in KRG-treated DM renal cells compared to DM renal cells. In addition, we found that mRNAs expression shows anti-correlation with miRNAs expression and further validated their biological processes and signaling pathways by Gene Ontology (GO). Conclusion Our data suggest that KRG may regulate miRNA and gene expression in a way that relieves diabetic nephropathy in mice.