Neurophysiological analysis of disadvantageous social inequity: Exploring emotional behavior changes and c-Fos expression in a male rat model

• Published in: Behavioural brain research Vol. 466; p. 114983
• Main Authors: Jeong, Yujeong, Noh, Jihyun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 28.05.2024
• Subjects: Animals; Anxiety; Behavior, Animal - physiology; Cortex; Cues; Emotions - physiology; Inequity aversion; Male; Nucleus accumbens; Nucleus Accumbens - metabolism; Prefrontal Cortex - metabolism; Proto-Oncogene Proteins c-fos - metabolism; Rats; Rats, Sprague-Dawley; Restraint stress; Reward; Social Behavior; Social interaction; Stress, Psychological - metabolism; Stress, Psychological - physiopathology; DE; LO; DI; NAcs; OFC; Inequity aversion; Restraint stress; ANOVA; IA; Anxiety; OFT; PBS; ACC; IL; AE; Cortex; AI; ACCv; DMS; Nucleus accumbens; Social interaction; BLA; NAcc; PL; ACCd; VMS; CeA
• ISSN: 0166-4328; 1872-7549; 1872-7549
• DOI: 10.1016/j.bbr.2024.114983

Humans and other animals exhibit aversive behavioral and emotional responses to unequal reward distributions compared with their conspecifics. Despite the significance of this phenomenon, experimental animal models designed to investigate social inequity aversion and delve into the underlying neurophysiological mechanisms are limited. In this study, we developed a rat model to determine the effects of socially equal or unequal reward and stress on emotional changes in male rats. During the training session, the rats were trained to escape when a sound cue was presented, and they were assigned to one of the following groups: all escaping rats [advantageous equity (AE)], freely moving rats alongside a restrained rat [advantageous inequity (AI)], all restrained rats [disadvantageous equity (DE)], and a rat restrained in the presence of freely moving companions [disadvantageous inequity (DI)]. During the test session, rats in the advantageous group (AE and AI) escaped after the cue sound (expected reward acquisition), whereas rats in the disadvantageous group (DE and DI) could not escape despite the cue being presented (expected reward deprivation). Emotional alteration induced by exposure to restraint stress under various social interaction circumstances was examined using an open field test. Notably, the DI group displayed reduced exploration of the center zone during the open field tests compared with the other groups, indicating heightened anxiety-like behaviors in response to reward inequity. Immunohistochemical analysis revealed increased c-Fos expression in the medial prefrontal and orbitofrontal cortices, coupled with reduced c-Fos expression in the striatum and nucleus accumbens under DI conditions, in contrast to the other experimental conditions. These findings provide compelling evidence that rats are particularly sensitive to reward inequity, shedding light on the neurophysiological basis for distinct cognitive processes that manifest when individuals are exposed to social equity and inequity situations. •Rats showed a social inequity aversive behavior.•The inequity group showed higher anxiety-like behaviors than others.•The inequity group had lower c-Fos expression in the striatum and NAc.•The inequity group showed higher c-Fos expression in the mPFC and OFC.