The Arabidopsis Anaphase-Promoting Complex/Cyclosome Subunit 1 is Critical for Both Female Gametogenesis and Embryogenesis

• Published in: Journal of integrative plant biology Vol. 55; no. 1; pp. 64 - 74
• Main Authors: Wang, Yanbing, Hou, Yingnan, Gu, Hongya, Kang, Dingming, Chen, Zhang-Liang, Liu, Jingjing, Qu, Li-Jia
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 2013; The National Plant Gene Research Center, Beijing 100101, China%College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China; State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China; College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China%State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China%State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China
• Subjects: Anaphase-Promoting Complex-Cyclosome; anaphase-promoting complex/cyclosome (APC/C); APC1; APC4; Arabidopsis; Arabidopsis - embryology; Arabidopsis - genetics; Arabidopsis - physiology; embryogenesis; female gametogenesis; Fertility; Glucuronidase - metabolism; Mutation; Ovule - enzymology; Ovule - growth & development; Seeds - growth & development; synergistic role; T-DNA插入; Ubiquitin-Protein Ligase Complexes - physiology; 亚基; 后期; 拟南芥; 细胞周期蛋白B; 细胞周期调控; 胚胎发育过程; 配子发生; synergistic role; APC1; embryogenesis; Arabidopsis; female gametogenesis; APC4; anaphase-promoting complex/cyclosome (APC/C)
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/jipb.12018

Anaphase-promoting complex/cyclosome （APC/C）, a multisubunit E3 ligase, plays a critical role in cell cycle control, but the functional characterization of each subunit has not yet been completed. To investigate the function of APC1 in Arabidopsis, we analyzed four mutant alleles of APC1, and found that mutation in APC1 resulted in significantly reduced plant fertility, accumulation of cyclin B, and disrupted auxin distribution in embryos. The three mutant alleles apcl-1, apcl-2 and apcl-3 shared variable defects in female gametogenesis including degradation, abnormal nuclear number, and disrupted polarity of nuclei in the embryo sac as well as in embryogenesis, in which embryos were arrested at multiple stages. All of these defects are similar to those previously identified in apc4. The mutant apcl-4, in which the T-DNA was inserted after the transmembrane domain at the C-terminus, showed much more severe phenotypes; that is, most of the ovules were arrested at the one-nucleate female gametophyte stage （stage FG1）. In the apcl apc4 double mutants, the fertility was further reduced by one-third in apcl-ll＋ apc4-1/＋, and in some cases no ovules even survived in siliques of apcl-4/＋ apc4-1/＋. Our data thus suggest that APC1, an essential component of APC/C, plays a synergistic role with APC4 both in female gametogenesis and in embryogenesis.