Vascular endothelial growth factor polymorphisms in mantle cell lymphoma

• Published in: Acta haematologica Vol. 123; no. 2; p. 91
• Main Authors: Galimberti, S, Nagy, B, Palumbo, G A, Ciancia, E, Buda, G, Orciuolo, E, Melosi, A, Lambelet, P, Ronca, F, Petrini, M
• Format: Journal Article
• Language: English
• Published: Switzerland 01.01.2010
• Subjects: Adult; Aged; Female; Gene Frequency; Humans; Lymphoma, Mantle-Cell - genetics; Male; Middle Aged; Polymorphism, Single Nucleotide; Vascular Endothelial Growth Factor A - genetics
• ISSN: 1421-9662
• DOI: 10.1159/000267826

In this study, we determined the allele and genotype frequencies of vascular endothelial growth factor (VEGF) G+405C, C-460T, C+936T and C-2578A single nucleotide polymorphisms (SNPs) in 32 patients affected by mantle cell lymphoma (MCL) and 58 healthy controls. Real-time PCR combined with melting curve analysis was used for the determination of SNP alleles. A significant difference in the allele frequency of VEGFC-460T and C+936T SNPs in MCL and healthy cases was not observed. On the contrary, VEGF G+405C and C-2578A SNP allele distribution was significantly lower in the patient group than among normal controls (p = 0.014, p = 0.001). This observation suggests that further investigation is warranted, both in vitro and in a larger series of patients, to further examine the role of VEGF polymorphisms in the pathogenesis of MCL. In addition, the use of quantitative real-time PCR combined with a melting curve analysis method in the detection of the 4 VEGF SNPs may have the potential to replace older and more time-consuming PCR-RFLP methods and bears further investigation.