Production of macrophage inhibition factor by rat lymphocytes following exposure to isologous liver subcellular membranes

• Published in: Clinical immunology and immunopathology Vol. 3; no. 4; pp. 541 - 554
• Main Authors: McCalmon, Robert T., Pinckard, R.Neal, Olson, Merle S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.1975
• Subjects: Animals; Cell Membrane - immunology; Cell Migration Inhibition; Cells, Cultured; Centrifugation, Density Gradient; Chemical Fractionation; Concanavalin A; Electron Transport Complex IV - analysis; Female; Freund's Adjuvant; Glucosephosphate Dehydrogenase - analysis; Immunization; Lectins; Liver - ultrastructure; Lymph Nodes - cytology; Lymphocytes - immunology; Macrophage Migration-Inhibitory Factors - biosynthesis; Macrophages - immunology; Mitochondria, Liver - immunology; Molecular Weight; Rats; Rats, Inbred Lew; Subcellular Fractions - enzymology; Thymidine - metabolism; Thymus Gland - immunology; Tritium; Tuberculin
• ISSN: 0090-1229; 1090-2341
• DOI: 10.1016/0090-1229(75)90079-3

Direct inhibition of marcophage migration occurred when rat peritoneal exudate cells obtained from syngeneic Lewis rats were incubated with isologous rat liver membranous subcellular constituents. Subcellular membranes also induced the release of a migration inhibition factor (MIF) from isologous lymph node cells. Mitogenic activity was not detected upon incubation of lymph node cells with subcellular membranes. However, both the mitochondria-induced direct inhibition of macrophage migration and the production of MIF by lymph node cells were shown to be thymic dependent.