Ultraviolet absorption spectroscopy of peptides

UV absorption spectroscopy is commonly used with peptides for determining concentration and enzyme activity, but high-resolution UV spectra can also provide information on peptide secondary and tertiary structure and association behavior. New developments using temperature- and cation-dependent high...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 1088; p. 225
Main Authors Liyanage, Mangala R, Bakshi, Kunal, Volkin, David B, Middaugh, C Russell
Format Journal Article
LanguageEnglish
Published United States 01.01.2014
Subjects
Absorption
Data Interpretation, Statistical
Kinetics
Peptides - chemistry
Spectrophotometry, Ultraviolet - methods
Temperature
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Summary:UV absorption spectroscopy is commonly used with peptides for determining concentration and enzyme activity, but high-resolution UV spectra can also provide information on peptide secondary and tertiary structure and association behavior. New developments using temperature- and cation-dependent high-resolution second derivative absorption methods can also provide information concerning peptide dynamics. Data from several low-resolution spectroscopic techniques, including UV absorption, can be combined to generate an overall picture of peptide structure as a function of environmental conditions.
ISSN:1940-6029
DOI:10.1007/978-1-62703-673-3_15