Ultraviolet absorption spectroscopy of peptides
UV absorption spectroscopy is commonly used with peptides for determining concentration and enzyme activity, but high-resolution UV spectra can also provide information on peptide secondary and tertiary structure and association behavior. New developments using temperature- and cation-dependent high...
Saved in:
Published in | Methods in molecular biology (Clifton, N.J.) Vol. 1088; p. 225 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.01.2014
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Summary: | UV absorption spectroscopy is commonly used with peptides for determining concentration and enzyme activity, but high-resolution UV spectra can also provide information on peptide secondary and tertiary structure and association behavior. New developments using temperature- and cation-dependent high-resolution second derivative absorption methods can also provide information concerning peptide dynamics. Data from several low-resolution spectroscopic techniques, including UV absorption, can be combined to generate an overall picture of peptide structure as a function of environmental conditions. |
---|---|
ISSN: | 1940-6029 |
DOI: | 10.1007/978-1-62703-673-3_15 |