Oral administration of Synechococcus sp. PCC7942 trans-vp19 and trans-vp (19+28) genes improve the immune and antioxidant capacity in Procambarus clarkii under white spot syndrome virus stress

• Published in: Fish & shellfish immunology Vol. 132; p. 108471
• Main Authors: Yang, Jia, Chi, Xiao-Ping, Li, Man, He, Wen-hui, Jia, Rui, He, Pei-Min
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2023
• Subjects: Administration, Oral; Animals; Antioxidants; Astacoidea; Enzyme activities; Procambarus clarkii; Synechococcus - genetics; Synechococcus sp. PCC7942; vp (19+28); vp19; White spot syndrome virus (WSSV); White spot syndrome virus 1 - physiology; Enzyme activities; Synechococcus sp. PCC7942; Procambarus clarkii; vp (19+28); vp19; White spot syndrome virus (WSSV)
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2022.108471

This study aimed to evaluate antioxidant capacity and protection from white spot syndrome virus (WSSV) challenge of Procambarus clarkii fed trans-vp19 and trans-vp (19 + 28) genes of Synechococcus sp. PCC7942 (Syn7942). P. clarkii were fed transgenic cyanobacteria continuously for 7 days, and then infected with WSSV after 12 h starvation. The daily mortality in each group was measured for 10 days and hepatopancreas and muscle of P. clarkii were examined for enzymes phenoloxidase (PO) activity, catalase (CAT) activity, glutathione peroxidase (GSH-px) activity, and malondialdehyde (MDA) concentration after immunization and viral challenge at different times. Compared with the WSSV-infected crayfish in positive control group (challenge and no vaccination) and wild type group (challenge, feeding wild-type Syn7942), vp19 group (challenge, feeding Syn7942 trans-vp19 gene) and vp (19 + 28) group [challenge, feeding Syn7942 trans-vp (19 + 28) genes] significantly improved the survival rate from 0% to 60% and 56.7%, respectively. Consistently, significantly greater PO, CAT, and GSH-px activity and significantly lower MDA concentration in the vp19 and vp (19 + 28) groups compared to the control group. These results demonstrate that the trans-vp19 and trans-vp (19 + 28) gene of Syn7942 significantly facilitated the immune and antioxidant capacity of crayfish. Therefore, the trans-vp19 and trans-vp (19 + 28) genes of Syn7942 could provide protection for crayfish as an anti-WSSV oral medication. •First use of Synechococcus sp. PCC7942 trans-vp19 and vp (19 + 28) genes in crayfish against white spot syndrome virus.•Transgenic cyanobacteria can effectively improve the survival rate of crayfish infected with WSSV.•Transgenic cyanobacteria can improve the activity of immune enzymes and reduce the accumulation of MDA in crayfish.