Branched oligonucleotides containing bicyclic nucleotides as branching points and DNA or LNA as triplex forming branch

• Published in: Bioorganic & medicinal chemistry letters Vol. 10; no. 16; pp. 1853 - 1856
• Main Authors: Sørensen, Mads D, Meldgaard, Michael, Rajwanshi, Vivek K, Wengel, Jesper
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 21.08.2000; Elsevier
• Subjects: Base Pairing; Bridged Bicyclo Compounds - chemical synthesis; Bridged Bicyclo Compounds - chemistry; Bridged Bicyclo Compounds - metabolism; Carbohydrates. Nucleosides and nucleotides; Chemistry; DNA - chemistry; DNA - metabolism; Exact sciences and technology; Molecular Structure; Nucleic Acid Conformation; Nucleic Acid Hybridization; Nucleosides, nucleotides and oligonucleotides; Oligonucleotides - chemical synthesis; Oligonucleotides - chemistry; Oligonucleotides - metabolism; Organic chemistry; Preparations and properties; Molecular rigidity; Molecular hybridization; Automation; Triplet; Organic phosphoramidite; Branched compound; Oxygen heterocycle; Antisense oligonucleotide; Base pairing; Thermal stability; DNA; Bicyclic compound; Solid phase; Chemical synthesis
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/S0960-894X(00)00362-0

Various Y-shaped branched oligonucleotides containing a 2′- O,3′- C-ethylene linked or 2′- O,4′- C-methylene linked bicyclic nucleotide as branching point were synthesized on an automated DNA synthesizer. Thermal denaturation experiments at 260 and 284 nm showed increased thermal stabilities of complexes formed between these Y-shaped oligonucleotides and complementary DNA compared with those formed with the corresponding linear reference. The most significant effect was observed when LNA (locked nucleic acid) monomers were used in the triplex forming branch.