In vivo and in vitro monitoring of phagosome maturation in retinal pigment epithelium cells

The ingestion and degradation of photoreceptor disk membranes is a critical and major role for the retinal pigment epithelium (RPE). To help elucidate the cellular events involved in this role, functional in vivo and in vitro assays need to be developed further. Here we propose a method to help moni...

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Bibliographic Details
Published inAdvances in experimental medicine and biology Vol. 801; p. 85
Main Authors Esteve-Rudd, Julian, Lopes, Vanda S, Jiang, Mei, Williams, David S
Format Journal Article
LanguageEnglish
Published United States 2014
Subjects
Animals
Antibody Specificity
Cells, Cultured
In Vitro Techniques
Mice
Opsins - immunology
Opsins - metabolism
Phagosomes - physiology
Phagosomes - ultrastructure
Retinal Photoreceptor Cell Outer Segment - physiology
Retinal Pigment Epithelium - cytology
Retinal Pigment Epithelium - physiology
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Summary:The ingestion and degradation of photoreceptor disk membranes is a critical and major role for the retinal pigment epithelium (RPE). To help elucidate the cellular events involved in this role, functional in vivo and in vitro assays need to be developed further. Here we propose a method to help monitor phagosome maturation, using antibodies against different epitopes of opsin. We show that antibodies specific for the C-terminus of opsin label only immature phagosomes located in the apical region of the RPE. In contrast, antibodies recognizing the N-terminus also label more mature phagosomes, located more basally. The combined use of antibodies against different opsin epitopes thus provides a valuable tool in the study of phagosome maturation in the RPE.
ISSN:0065-2598
DOI:10.1007/978-1-4614-3209-8_11