Peroxidase-like activity and amperometric sensing of hydrogen peroxide by Fe2O3 and Prussian Blue-modified Fe2O3 nanoparticles

• Published in: Journal of molecular catalysis. A, Chemical Vol. 360; pp. 71 - 77
• Main Authors: Dutta, Amit Kumar, Maji, Swarup Kumar, Srivastava, Divesh N., Mondal, Anup, Biswas, Papu, Paul, Parimal, Adhikary, Bibhutosh
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2012
• Subjects: Amperometric sensor; Electrocatalytic activity; Fe2O3 nanoparticles; Peroxidase mimic; Prussian Blue modified Fe2O3; Prussian Blue modified Fe2O3; Electrocatalytic activity; Amperometric sensor; Peroxidase mimic; Fe2O3 nanoparticles
• ISSN: 1381-1169; 1873-314X
• DOI: 10.1016/j.molcata.2012.04.011

[Display omitted] ► Fe2O3 NPs synthesized from a single-source precursor and modified with Prussian Blue. ► The Fe2O3 and PB-Fe2O3 characterized by XRD, TEM, UV–vis and IR spectral techniques. ► Catalytic activity toward peroxidase substrate TMB oxidation in presence of H2O2. ► PB-Fe2O3 immobilized on GCE, used for amperometric detection of H2O2. Nano-sized crystals of iron oxide (Fe2O3) was synthesized from a single-source precursor complex [Fe3(μ3-O)(O2CCH2Cl)6(H2O)3]NO3·H2O by solvothermal process. Afterward it was chemically modified with electro-active Prussian Blue (PB). The resulting Fe2O3 and PB-Fe2O3 nanoparticles (NPs) were characterized by X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), UV–vis and IR spectroscopic techniques. Structural analyses showed that the average diameter of hexagonal Fe2O3 (maghemite) and PB-Fe2O3 NPs are 40 and 46nm, respectively. The as-synthesized nanocomposite (PB-Fe2O3 NPs) showed superior catalytic activity toward oxidation of peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in presence of H2O2 and follow typical Michaelis–Menten kinetics. Furthermore, the nanocomposite, immobilized on surface of a glassy carbon electrode (GCE), exhibited electrocatalytic activity toward reduction of hydrogen peroxide and can be used for its amperometric detection.