Mouse Models of Melanoma Bone Metastasis

Melanomas are aggressive cancers of the skin with high metastatic capacity. Mouse models are necessary to delineate the mechanisms of cancer metastasis and xenograft models can also allow examining the role of the host using different genetically-modified mouse models. In this chapter, I provide a d...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1914; p. 343
Main Author Uluçkan, Özge
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Bone metastasis
Firefly luciferase
B16 cells
Imaging
Melanoma
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Summary:Melanomas are aggressive cancers of the skin with high metastatic capacity. Mouse models are necessary to delineate the mechanisms of cancer metastasis and xenograft models can also allow examining the role of the host using different genetically-modified mouse models. In this chapter, I provide a detailed protocol for the preparation and inoculation of tumor cells intra-cardially and intra-tibially to achieve bone metastasis.
ISSN:1940-6029
DOI:10.1007/978-1-4939-8997-3_19