Transcriptional responses of Crassostrea hongkongensis under high and low salinity stress

• Published in: Comparative biochemistry and physiology. Part D, Genomics & proteomics Vol. 49; p. 101188
• Main Authors: Li, Wei, Zhang, Xingzhi, He, Pingping, Jiang, Linyuan, Zhang, Li, Guan, Junliang, Chen, Yongxian, Zheng, Yusi, Wei, Pinyuan, Peng, Jinxia
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.03.2024
• Subjects: Animals; Arginine - genetics; Arginine - metabolism; Crassostrea - metabolism; Crassostrea hongkongensis; Proline - genetics; Proline - metabolism; RT-qPCR; Salinity; Salinity stress; Salt Stress; Transcriptome; Salinity stress; Crassostrea hongkongensis; RT-qPCR; Transcriptome
• ISSN: 1744-117X; 1878-0407
• DOI: 10.1016/j.cbd.2024.101188

Salinity, a key limiting factor, affects the distribution and survival of marine species. The Hong Kong oyster (Crassostrea hongkongensis), a euryhaline species found along the coast of the South China Sea, has become a major aquaculture bivalve species. To determine the molecular mechanism by which oysters respond to coastal waters with varying salinity levels, we used RNA-seq to sequence the gill samples of oysters exposed to normal (25 ‰, S25), low (5 ‰, S5) and high (35 ‰, S35) salinity conditions for one month. The results revealed different expression transcriptome levels among oysters living under low and high salinity conditions. Using high-throughput sequencing, we identified 811 up-regulated genes and 769 down-regulated genes. As determined by KEGG pathway mapping, the differentially expressed genes (DEGs) were significantly enriched in the prion diseases, histidine metabolism, arginine and proline metabolism, and beta-alanine metabolism pathways in both the S5 vs. S25 and S35 vs. S25 group comparison. Several DEGs including heat shock 70 kDa protein 12B-like, poly (ADP-ribose) polymerase (PARP), and tripartite motif-containing protein 2 (TRIM2), and low-density lipoprotein receptor-like, as well as KEGG pathways, including arginine and proline metabolism, apoptosis, PPAR signaling pathway, the thyroid hormone signaling pathway, were concerning response to salinity stress. Additionally, eight DEGs involved in salinity adaptation were selected for RT-qPCR validation, and the results confirmed the credibility of the transcriptome sequencing data. Overall, we designed a one-month, medium-term experiment to examine the responses of C. hongkongensis exposed to different levels of salinity stress and performed transcriptome analysis using high-throughput sequencing. Our results enhance current understanding of the molecular mechanisms of salinity stress responses in C. hongkongensis and provided insights into the osmotic biology of oysters. •We performed a transcriptome analysis of Crassostrea hongkongensis exposed to salinity stress.•Totals of 1303 and 277 DEGs were identified in the S5 vs. S25 and S35 vs. S25 group, respectively.•The DEGs were significantly enriched in KEGG pathways associated with salinity adaptation.•This study contributes to exploring molecular mechanism of salinity stress in C. hongkongensis.