Effect of Transglutaminase on the Properties of Films Prepared from Chitosan and Gelatin

• Published in: Applied biochemistry and microbiology Vol. 57; no. 3; pp. 366 - 372
• Main Authors: Kadimaliev, D. A., Parchaykina, O. V., Syusin, I. V., Chairkin, I. V., Malafeev, A. N., Devyatkin, A. A., Revin, V. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.05.2021; Springer Nature B.V
• Subjects: Amino groups; Atomic force microscopy; Biochemistry; Biodegradability; Biodegradation; Biomedical and Life Sciences; Chitosan; Crosslinking; Electron microscopy; Gelatin; Gels; Glycerol; Infrared spectroscopy; Life Sciences; Maltodextrin; Medical materials; Medical Microbiology; Microbiology; Microorganisms; Tensile strength; Transglutaminase 2; Wound healing; polymer films; chitosan; transglutaminase; cross-linking; gelatin; physical and mechanical properties
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683821030042

The production conditions were optimized, and the characteristics of biodegradable chitosan-gelatin films crosslinked with various microbial transglutaminases (transglutaminase 1, which contains maltodextrin, and transglutaminase 2, which contains maltodextrin, a milk protein) were studied. The film properties depended on both the cross-linking conditions and the type and ratio of components in the mixture. Gels and films prepared from chitosan and gelatin with transglutaminase 1 had very low characteristics as compared to films obtained with transglutaminase 2. Infrared spectroscopy, X-ray tomography, and scanning atomic force electron microscopy showed that transglutaminase 2 forms cross-links between both gelatin and chitosan amino groups, which improves the film characteristics. Films prepared from chitosan, gelatin, and glycerol crosslinked with transglutaminase 2 had the following characteristics: a strength of 15.8 MPa, a tensile strength of 220.0%, a thickness of 73.1 μm, and a density of 1.9 g/cm 3 . The biodegradability of the films depended on the composition and ranged from 20 to 30 days. The resulting films can serve as a matrix to obtain medical materials with antibacterial and wound-healing properties.