Microsatellite primers for Texas wild rice (Zizania texana), and a preliminary test of the impact of cryogenic storage on allele frequency at these loci

Seed collections in gene banks are useful for the preservation of wild germplasm, providing inexpensive insurance for species that survive in conventional cold storage (-18 °C). Seeds that cannot survive these conditions must be pretreated with cryoprotectants and stored at liquid nitrogen temperatu...

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Published inConservation genetics Vol. 5; no. 6; pp. 853 - 859
Main Authors Richards, Christopher M, Reilley, Ann, Touchell, Darren, Antolin, Michael F, Walters, Christina
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.11.2004
Subjects
Animal populations
Cold storage
Conservation genetics
Cryogenics
Cryopreservation
Cryoprotectors
Embryos
Gene banks
Gene frequency
Genetic diversity
Genetic markers
Germplasm
Grasses
Microsatellites
Nitrogen
Oryza sativa
Primers
Seeds
Statistics
Temperature effects
Zizania texana
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Summary:Seed collections in gene banks are useful for the preservation of wild germplasm, providing inexpensive insurance for species that survive in conventional cold storage (-18 °C). Seeds that cannot survive these conditions must be pretreated with cryoprotectants and stored at liquid nitrogen temperatures, which presents unique technical and methodological challenges. Implicit in this approach is the assumption that these added manipulations do not change the genetic diversity of the preserved collections. We used polymorphic microsatellite markers for an endangered aquatic grass, Texas wild rice (Zizania texana), to conduct a preliminary evaluation of the effects of cryogenic preservation of mature embryos on genetic diversity. Using several statistical approaches, we show that allele frequencies did not change in collections of seeds that underwent cryopreservation (cryoprotected) compared to those samples that was not exposed to cryopreservation (control). The retention of the allelic diversity at the five loci examined suggests that there were no significant changes in genetic diversity due to treatments and that these protocols may be appropriate for ex situ conservation of genetically diverse wild germplasm.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:1566-0621
1572-9737
DOI:10.1007/s10592-004-1977-3