An established kidney cell line from humpback grouper (Cromileptes altivelis) and its susceptibility to bacteria and heavy metals

• Published in: Fish physiology and biochemistry Vol. 48; no. 3; pp. 521 - 533
• Main Authors: Wei, Caoying, Yang, Xin, Kang, Minjie, Cao, Zhenjie, Sun, Yun, Zhou, Yongcan
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2022; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Animal Physiology; Aquaculture; Aquaculture development; Bacteria; Biomedical and Life Sciences; Breeding; Cadmium; Calloplesiops altivelis; Cells; Chromosomes; Copper; Cromileptes altivelis; Cytotoxicity; Economics; Efficiency; Fetal calf serum; Fish; Freshwater & Marine Ecology; Genes; Genomics; Heavy metals; Histology; Industrial development; Infections; Kidneys; Laboratories; Life Sciences; Marine aquaculture; Marine fishes; Mercury; Metals; Morphology; Pathogens; Plasmids; Pollutants; rRNA 18S; Serum; Susceptibility; Toxicity; Toxicology; Transfection; Trout; Zoology; Kidney cell line; Cytotoxicity; Transfection efficiency; Bacterial susceptibility; Cromileptes altivelis
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-022-01065-5

Humpback grouper ( Cromileptes altivelis ), one kind of commercial fish with considerable economic value, has been recognized as a promising candidate for mariculture. In the wake of the development of aquaculture industry, the breeding density of C. altivelis has increased gradually, which gave rise to the occurrence of various pathogenic diseases. In our research, we established a new kidney cell line (designated as CAK) from humpback grouper and evaluated its susceptibility to bacteria and heavy metals. The results of our study showed that the optimal growth temperature was 26 °C, and optimal medium was L-15 supplemented with 20% fetal bovine serum (FBS). The sequencing of 18S rRNA gene indicated that CAK cell line was derived from C. altivelis . Chromosome analysis showed that the number of chromosome in CAK was 48. After being transfected of pEGFP-N3 plasmid, high transfection efficiency of CAK was observed, suggesting the potential to be used for the study of foreign functional genes. Moreover, the bacterial susceptibility results revealed that CAK cells were sensitive to Vibrio harveyi and Edwardsiella tarda , especially V. harveyi . Meanwhile, three heavy metals (Hg, Cu, and Cd) had toxic effects on the CAK cells with a dose-dependent manner. To sum up, the CAK cell line might be an ideal tool in vitro for analyzing the function of exogenous genes, bacterial susceptibility, and toxicity assay of heavy metals.