Fluorescence spectroscopy of peptides

In peptides, steady-state fluorescence can be used to measure the intrinsic fluorescence of Trp, Tyr, and Phe residues, as well as associated dyes, which can change upon exposure to different environmental conditions. This technique can be thus used to detect changes in the conformational states of...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1088; p. 237
Main Authors Liyanage, Mangala R, Bakshi, Kunal, Volkin, David B, Middaugh, C Russell
Format Journal Article
LanguageEnglish
Published United States 01.01.2014
Subjects
alpha-MSH - chemistry
Angiotensin I - chemistry
Peptides - chemistry
Spectrometry, Fluorescence - methods
Statistics as Topic
Substance P - chemistry
Time Factors
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Summary:In peptides, steady-state fluorescence can be used to measure the intrinsic fluorescence of Trp, Tyr, and Phe residues, as well as associated dyes, which can change upon exposure to different environmental conditions. This technique can be thus used to detect changes in the conformational states of peptides. Time-resolved fluorescence can also be used to study fast motions of peptides and their interactions with fluorescence dyes. Data from several low-resolution spectroscopic techniques, including fluorescence, can be combined to generate an overall picture of peptide structure as a function of environmental conditions.
ISSN:1940-6029
DOI:10.1007/978-1-62703-673-3_16