A simple and accurate mathematical method for calculation of the EC50

A simple, accurate, and speedy noncomputational technique for the calculation of the EC50 or any other concentration-related parameter of concentration-effect curves is presented. It avoids the necessity for graph construction or computational curve-fitting programs and allows accurate calculation o...

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Bibliographic Details
Published inJournal of pharmacological and toxicological methods Vol. 41; no. 2-3; pp. 55 - 58
Main Authors Alexander, B, Browse, D J, Reading, S J, Benjamin, I S
Format Journal Article
LanguageEnglish
Published United States 01.04.1999
Subjects
Animals
Dose-Response Relationship, Drug
Hepatic Artery - metabolism
Humans
Lethal Dose 50
Liver - metabolism
Liver Circulation
Models, Theoretical
Norepinephrine - pharmacokinetics
Perfusion
Rats
Vasoconstrictor Agents - pharmacokinetics
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Summary:A simple, accurate, and speedy noncomputational technique for the calculation of the EC50 or any other concentration-related parameter of concentration-effect curves is presented. It avoids the necessity for graph construction or computational curve-fitting programs and allows accurate calculation of the EC50, where the value falls between two known concentrations The technique has been applied to a concentration-response curve constructed to hepatic arterial (HA) vasoconstrictor responses to HA injections of noradrenaline in an isolated dual-perfused rat liver preparation. EC50 values calculated by the new technique were compared to those calculated by conventional, established, noncomputational techniques. The new technique is faster, more accurate, and simpler to perform than other established noncomputational techniques used for the calculation of the EC50 and can be widely applied to many other pharmacological investigations.
ISSN:1056-8719
1873-488X
DOI:10.1016/S1056-8719(98)00038-0