Incorporation of single nucleoside triphosphates by isolated cell nuclei

Manganese ions can form insoluble complexes or coprecipitates with nucleoside triphosphates at high pH. We have demonstrated that nucleoside triphosphates found in precipitated complexes exhibit properties similar to those of poliribo-nucleotides in all steps of the Schmidt-Tannhausers procedure: in...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 83; no. 2; pp. 689 - 693
Main Authors Wojcierowski, J., Antosz, Halina
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.12.1977
Subjects
Animals
Cell Nucleus - metabolism
Kinetics
Liver - metabolism
Rats
Ribonucleotides - metabolism
RNA - biosynthesis
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Summary:Manganese ions can form insoluble complexes or coprecipitates with nucleoside triphosphates at high pH. We have demonstrated that nucleoside triphosphates found in precipitated complexes exhibit properties similar to those of poliribo-nucleotides in all steps of the Schmidt-Tannhausers procedure: insoluble in perchloric acid, trichloroacetic acid, and ethanol and soluble in 0.2 n NaOH. These observations could be applied to experimentation on RNA synthesis from nucleoside triphosphates.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(77)90073-2