Encapsulation of cytochrome c in hollow mesoporous silica spheres by denaturation

• Published in: Microporous and mesoporous materials Vol. 377; p. 113216
• Main Authors: Okamoto, Masaki, Naito, Yuki, Yamazaki, Kiyoyuki, Odai, Shunsuke, Ito, Hidehiro, Kamachi, Toshiaki
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.09.2024
• Subjects: Cytochrome c; Denaturation; Encapsulation; Hollow mesoporous silica sphere; Oxidation catalyst; Encapsulation; Cytochrome c; Hollow mesoporous silica sphere; Denaturation; Oxidation catalyst
• ISSN: 1387-1811
• DOI: 10.1016/j.micromeso.2024.113216

Cytochrome c (Cyt-c) was encapsulated in hollow mesoporous silica spheres as a support for immobilization. Cyt-c was denatured by an aqueous solution of guanidine hydrochloride to increase the flexibility of Cyt-c structure by unfolding and subsequently passed through the mesopores into the hollow spaces by diffusion. After incorporation, the refolding of Cyt-c was carried out by removing the guanidine hydrochloride using dialysis. Considering that the mesopore size was slightly smaller than the native Cyt-c size, Cyt-c was immobilized inside the hollow spheres. Modification of encapsulated Cyt-c with 18-crown-6 showed catalytic activity for the asymmetric oxidation of methyl 4-tolyl sulfoxide. The encapsulated catalyst could be reused to achieve almost the same catalytic activity. [Display omitted] •Cytochrome c was incorporated into hollow mesoporous silica spheres by diffusion of denatured cytochrome c through the mesopores.•Cytochrome c was encapsulated in hollow mesoporous silica spheres by restoring the native structure of cytochrome c through the removal of denaturants by dialysis.•Modified cytochrome c in hollow mesoporous silica spheres showed catalytic activity for asymmetric sulfoxide oxidation.