A unique implementation of Hantzsch reaction for determination of natamycin in Yoghurt: Hyphenated with Box-Behnken-design for optimization

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 317; p. 124395
• Main Authors: Mohamed, Abobakr A., Abdel-Hakam Abbas, Khaled, Shaaban Abdelmontaleb, Hani, Hamed, Mohammed I.A., Mostafa, Islam M., Ahmed Elsayed, Mohamed
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.09.2024
• Subjects: E235; Fluorescence; Natamycin; Yoghurt; Fluorescence; Natamycin; Yoghurt; E235
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2024.124395

[Display omitted] •Fluorescence-method for specific determination of natamycin was designed.•Determination of the studied drug in yoghurt samples.•Facile application of the designed fluorescence-method.•The developed method can present a fascinating methodology for natamycin analysis. This study aims to develop a novel and selective method for the detection of natamycin (E235) in yoghurt. The suggested method adopts an application of Hantzsch reaction to turn on the fluorescence behavior of natamycin (blue fluorescence), allowing its sensitive and selective determination in yoghurt samples without any overlapping at 485 nm. The originality of the research lies in the fact that this application takes place for the first time, also the detection (LOD) and quantification (LOQ) limits were very low (0.02 and 0.06μg mL−1, respectively) with a linear concentration range of 0.1–1.0 μgmL-1. Moreover, the developed method was employed for the detection of E235 in yoghurt sample with a good recoveries (98.80 ± 1.20–99.20 ± 1.15 (%), over a concentration range of 0.5–1.0 μgmL-1, (LOD = 0.04 and LOQ = 0.12 μgmL-1). Furthermore, the specificity and convenient application of our intended method is an attempt to determine E235 in milk anddairy products with easily followable steps.