Proteomics analysis of differentially abundant proteins in the rohu kidney infected with Edwardsiella tarda

• Published in: Comparative biochemistry and physiology. Part D, Genomics & proteomics Vol. 50; p. 101221
• Main Authors: Pinto, Nevil, Nissa, Mehar Un, Yashwanth, B.S., Sathiyanarayanan, A., Pai, Medha Gayathri J., Srivastava, Sanjeeva, Goswami, Mukunda
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.06.2024
• Subjects: Animals; Cyprinidae - metabolism; Cyprinidae - microbiology; Edwardsiella tarda; Enterobacteriaceae Infections - microbiology; Fish Diseases - metabolism; Fish Diseases - microbiology; Fish Proteins - metabolism; Kidney - metabolism; Kidney - microbiology; Labeo rohita; Multiple reaction monitoring; Phagosome; Protein Interaction Maps; Protein-protein interaction; Proteome - analysis; Proteomics; Tandem Mass Spectrometry; Wound healing; Wound healing; Protein-protein interaction; Labeo rohita; Edwardsiella tarda; Multiple reaction monitoring; Proteomics; Phagosome
• ISSN: 1744-117X; 1878-0407
• DOI: 10.1016/j.cbd.2024.101221

Edwardsiella tarda (Et) is a zoonotic gram-negative pathogen with a diverse host range, including fish. However, the in-depth molecular mechanisms underlying the response of Labeo rohita (rohu) kidney to Et are poorly understood. A proteomic and histopathological analysis was performed for the rohu kidney after Et infection. The histopathology of the infected rohu kidney showed vacuolation and necrosis. After LC-MS/MS analysis, ~1240 proteins were identified with ≥2 unique peptides. A total of 96 differentially abundant proteins (DAPs) were observed between the control and Et infected group (ET). Metascape and STRING analysis were used for the gene ontology (GO), and protein-protein interaction network (PPI) for the significant pathways of DAPs. In PPI, low-abundant proteins were mapped to metabolic pathways and oxidative phosphorylation (cox5ab, uqcrfs1). High-abundance proteins were mapped to ribosomes (rplp2), protein process in the ER (hspa8), and immune system (ptgdsb.1, muc2). Our label-free proteomic approach in the rohu kidney revealed abundant enriched proteins involved in vesicle coat (ehd4), complement activation (c3a.1, c9, c7a), phagosome (thbs4, mapk1), metabolic reprogramming (hao1, glud1a), wound healing (vim, alox5), and the immune system (psap) after Et infection. A targeted proteomics approach of multiple reaction monitoring (MRM) validated the DAPs (nprl3, ambp, vmo1a, hspg2, muc2, hao1 and glud1a) between control and ET. Overall, the current analysis of histology and proteome in the rohu kidney provides comprehensive data on pathogenicity and the potential immune proteins against Et. [Display omitted] •Edwardsiella tarda (Et) affects rohu and disease mechanism is poorly understood.•Label-free quantification (LFQ) approach was performed for Et infected rohu kidney.•Quantitative proteomic profiling of rohu against Et showed 96 differentially abundant proteins.•Et impaired kidney proteins involved in renal metabolic, wound healing and immune mechanism.•The altered proteins validated using MRM and may serve as valuable markers of infection.