Incorporation of ammonium in amino acids by Trypanosoma cruzi

Ammonium ions were incorporated into L-glutamate and alpha-ketoglutarate in epimastigote forms of Trypanosoma cruzi through the following enzymatic systems: NADPH and NADH-dependent glutamate dehydrogenase, NADPH-dependent glutamate synthase, L-glutamine synthetase and NADH-dependent glutamate synth...

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Bibliographic Details
Published inThe Journal of parasitology Vol. 66; no. 2; p. 213
Main Authors Caldas, R A, Araújo, E F, Felix, C R, Roitman, I
Format Journal Article
LanguageEnglish
Published United States 01.04.1980
Subjects
Ammonia - metabolism
Animals
Glutamate Dehydrogenase - metabolism
Glutamate Synthase - metabolism
Glutamate-Ammonia Ligase - metabolism
Glutamates - biosynthesis
Glutamine - biosynthesis
Hydrogen-Ion Concentration
Kinetics
NAD - pharmacology
NADP - pharmacology
Trypanosoma cruzi - enzymology
Trypanosoma cruzi - metabolism
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Summary:Ammonium ions were incorporated into L-glutamate and alpha-ketoglutarate in epimastigote forms of Trypanosoma cruzi through the following enzymatic systems: NADPH and NADH-dependent glutamate dehydrogenase, NADPH-dependent glutamate synthase, L-glutamine synthetase and NADH-dependent glutamate synthase in order of decreasing specific activity (mumoles of product formed/min/mg protein). The pH optima and Km's for the glutamate dehydrogenase system were determined. Disc electrophoresis showed the presence of cathodic bands of GDH activity, which were highly dependent on NADP+.
ISSN:0022-3395
DOI:10.2307/3280806