A role of cell adhesion molecules and gelatinases in human serum-induced aggregation of human eyelid-derived stem cells in vitro
Human serum (HS) has been reported to induce aggregation of human eyelid adipose-derived stem cells (HEACs) during high-density culture in vitro. The present study focused on the role of cell adhesion molecules and gelatinases during HS-induced aggregation of HEACs. HS-induced aggregation occurred b...
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Published in | Balsaeng'gwa saengsig Vol. 17; no. 4; pp. 409 - 420 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Korea (South)
한국발생생물학회
01.12.2013
Korean Society of Developmental Biology |
Subjects | |
Online Access | Get full text |
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Summary: | Human serum (HS) has been reported to induce aggregation of human eyelid adipose-derived stem cells (HEACs) during high-density culture in vitro. The present study focused on the role of cell adhesion molecules and gelatinases during HS-induced aggregation of HEACs. HS-induced aggregation occurred between 9-15 days of culture. Cells aggregated by HS medium (HS-agg) showed stronger expression of α2, α2B, αX, and CEACAM1 genes compared to non-aggregated cells in HS medium (HS-ex) or in control FBS-cultured cells. HS-agg were distinctly labeled with antibodies against α2, α2B, and αX proteins. Western blot results demonstrated that the two integrin proteins were greatly expressed in HS-agg compared to HS-ex and control FBS-cultured cells. Treatment of HEACs with anti-integrin α2 antibody during culture in HS medium delayed aggregation formation. HS-agg exhibited strong expression of MMP1 and MMP9 compared to HS-ex or FBS-cultured cells. Conditioned media from HS-culture showed remarkable increase of MMP9 gelatinolytic activity in comparison to those from FBS-culture. However, there was no change of TIMP mRNA expression in relation to the HS-induced aggregation. Based on these results, it is suggested that integrin α2, α2B, and αX, and MMP9 might play an important role in the HS-induced aggregation of HEACs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 http://www.ksdb.org G704-001746.2013.17.4.019 |
ISSN: | 2465-9525 2465-9541 |
DOI: | 10.12717/DR.2013.17.4.409 |