UV-B Induced stimulation of phosphatidylinositol 3-kinase in human fetal hepatocytes

• Published in: Indian journal of clinical biochemistry Vol. 19; no. 1; pp. 118 - 121
• Main Authors: Chari, V B, Gaddameedi, R S R, Singh, M Raghuveer, Kasina, Sathish, Rahman, Khaja Khaleel Ur, Habibullah, C M, Singh, Surya S
• Format: Journal Article
• Language: English
• Published: India Springer Nature B.V 01.01.2004; Springer India
• Subjects: 1-Phosphatidylinositol 3-kinase; Cell survival; Cell viability; Fetuses; Hepatocytes; Lipid kinase; Proteins; Transplantation; Wortmannin; Signal transduction; Lipid kinase; Hepatocytes transplantation; Xenotransplantation
• ISSN: 0970-1915; 0974-0422
• DOI: 10.1007/BF02872405

We investigated the effect of UV-B irradiation on PI 3-kinase activity in human fetal hepatocytes. When cells were exposed to monochromatic (304nm) UV-B light, a significant increase in intracellular PI 3-kinase activity was observed in a dose dependent manner with maximal activity upon 1500 Jm(-2) irradiation. At 1500 Jm(-2) dose PI 3-kinse activity increased by 80% in membrane fraction of fetal hepatocytes of 25 weeks gestation. PI 3-kinse inhibitors wortmannin and LY294002 specifically inhibited the UV-B induced lipid kinase activity and blocked significantly the UV-B induced cell viability. The data suggests a correlation between cell survival and elevated levels of PI 3-kinase and suggest that UV-B irradiation at a dose of 1500 Jm(-2) is ideal for fetal hepatocyte transplantation. Also, PI 3-kinase levels could be a representative marker for viable UV-B irradiated fetal hepatocytes for transplantation.