Enhanced-reality video fluorescence: a real-time assessment of intestinal viability

• Published in: Annals of surgery Vol. 259; no. 4; p. 700
• Main Authors: Diana, Michele, Noll, Eric, Diemunsch, Pierre, Dallemagne, Bernard, Benahmed, Malika A, Agnus, Vincent, Soler, Luc, Barry, Brian, Namer, Izzie Jacques, Demartines, Nicolas, Charles, Anne-Laure, Geny, Bernard, Marescaux, Jacques
• Format: Journal Article
• Language: English
• Published: United States 01.04.2014
• Subjects: Animals; Biomarkers - metabolism; Female; Fluorescent Dyes; Image Interpretation, Computer-Assisted; Indocyanine Green; Intestine, Small - blood supply; Intestine, Small - metabolism; Intestine, Small - pathology; Ischemia - metabolism; Ischemia - pathology; Lactic Acid - metabolism; Laparoscopy; Magnetic Resonance Spectroscopy; Male; Mesenteric Arteries - surgery; Mesentery; Metabolome; Mitochondria - metabolism; Spectrometry, Fluorescence - methods; Swine
• ISSN: 1528-1140
• DOI: 10.1097/SLA.0b013e31828d4ab3

Our aim was to evaluate a fluorescence-based enhanced-reality system to assess intestinal viability in a laparoscopic mesenteric ischemia model. A small bowel loop was exposed, and 3 to 4 mesenteric vessels were clipped in 6 pigs. Indocyanine green (ICG) was administered intravenously 15 minutes later. The bowel was illuminated with an incoherent light source laparoscope (D-light-P, KarlStorz). The ICG fluorescence signal was analyzed with Ad Hoc imaging software (VR-RENDER), which provides a digital perfusion cartography that was superimposed to the intraoperative laparoscopic image [augmented reality (AR) synthesis]. Five regions of interest (ROIs) were marked under AR guidance (1, 2a-2b, 3a-3b corresponding to the ischemic, marginal, and vascularized zones, respectively). One hour later, capillary blood samples were obtained by puncturing the bowel serosa at the identified ROIs and lactates were measured using the EDGE analyzer. A surgical biopsy of each intestinal ROI was sent for mitochondrial respiratory rate assessment and for metabolites quantification. Mean capillary lactate levels were 3.98 (SD = 1.91) versus 1.05 (SD = 0.46) versus 0.74 (SD = 0.34) mmol/L at ROI 1 versus 2a-2b (P = 0.0001) versus 3a-3b (P = 0.0001), respectively. Mean maximal mitochondrial respiratory rate was 104.4 (±21.58) pmolO2/second/mg at the ROI 1 versus 191.1 ± 14.48 (2b, P = 0.03) versus 180.4 ± 16.71 (3a, P = 0.02) versus 199.2 ± 25.21 (3b, P = 0.02). Alanine, choline, ethanolamine, glucose, lactate, myoinositol, phosphocholine, sylloinositol, and valine showed statistically significant different concentrations between ischemic and nonischemic segments. Fluorescence-based AR may effectively detect the boundary between the ischemic and the vascularized zones in this experimental model.