In vivo biosynthetic studies of the Dolichos biflorus seed lectin

• Published in: Plant physiology (Bethesda) Vol. 91; no. 4; pp. 1382 - 1386
• Main Authors: Quinn, J.M. (University of California, Davis, CA), Etzler, M.E
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.12.1989
• Subjects: 550201 - Biochemistry- Tracer Techniques; AMINES; AMINOAZUCARES; AMINOSUCRE; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; CARBOHYDRATES; CARBON 14 COMPOUNDS; Cotyledons; DAYS LIVING RADIOISOTOPES; EVEN-ODD NUCLEI; EXPERIMENTACION IN VIVO; EXPERIMENTATION IN VIVO; Fundamental and applied biological sciences. Psychology; Gels; GENE; GENES; GLUCOSAMINE; GRAINE; HEXOSAMINES; HEXOSES; IN VIVO; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; LECTINA; LECTINE; LECTINS; LEGUMINOSAE; LIGHT NUCLEI; MAGNOLIOPHYTA; MAGNOLIOPSIDA; Metabolism; Metabolism. Physicochemical requirements; METHIONINE; METIONINA; MONOSACCHARIDES; NUCLEI; ORGANIC COMPOUNDS; Peas; Phytohemagglutinins; Plant physiology and development; PLANTS; Protein precursors; Proteins; RADIOISOTOPES; Resins; SACCHARIDES; Seeds; SEMILLA; SULFUR 35; SULFUR COMPOUNDS; SULFUR ISOTOPES; SYNTHESIS; TECHNIQUE DES TRACEURS; TECNICAS DE AISLAMIENTO DE ISOTOPOS; TRACER TECHNIQUES; Lectin; Seeds; Leguminosae; Radiolabelling; Dicotyledones; Angiospermae; Spermatophyta; Biosynthesis; Glycoproteins; Subunit
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.91.4.1382

The in vivo biosynthesis of the Dolichos biflorus seed lectin was studied by pulse-chase labeling experiments using [35S] methionine and [14C]glucosamine. These studies demonstrate that each of the two mature lectin subunit types are derived by the processing of separate glycosylated precursors. The appearance of the precursor to subunit I before the precursor to subunit II supports the possibility raised by previous studies that both subunit types of this lectin may originate from a single gene product