Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5

• Published in: Genomics (San Diego, Calif.) Vol. 14; no. 1; pp. 59 - 62
• Main Authors: Sieburth, D., Jabs, E.W., Warrington, J.A., Li, X., Lasota, J., LaForgia, S., Kelleher, K., Huebner, K., Wasmuth, J.J., Wolf, S.F.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.09.1992; Elsevier
• Subjects: Animals; Base Sequence; Biological and medical sciences; Blotting, Southern; Chromosomes, Human, Pair 3; Chromosomes, Human, Pair 5; Classical genetics, quantitative genetics, hybrids; Cricetinae; DNA - analysis; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; Hybrid Cells; Interleukin-12; Interleukins - genetics; Molecular Sequence Data; Oligonucleotide Probes; Polymerase Chain Reaction; Genetic mapping; Human; B-Lymphocyte; B5-Chromosome; A3-Chromosome; Cytokine
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/S0888-7543(05)80283-6

IL12 (formerly NKSF or CLMF) is a unique cytokine composed of two unrelated disulfide-linked subunits. The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF. The chromosomal localization of these two subunits was determined by PCR analysis of DNA from rodent-human hybrids. More refined mapping was obtained by PCR analysis of hybrids containing translocation chromosomes and for p40, by analysis of radiation hybrids. The subunits map to different chromosomes: p40 (IL12B) to 5q31–q33 and p35 (IL12A) to 3p12–3q13.2.