Adoptive transfer of murine experimental autoimmune encephalomyelitis in SJL. Thy-1 congenic mouse strains

• Published in: Journal of neuroimmunology Vol. 46; no. 1; pp. 129 - 136
• Main Authors: Kim, Changhee, Tse, Harley Y.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.07.1993
• Subjects: Adoptive transfer; Chronic relapsing experimental autoimmune encephalomyelitis; Congenic mouse strains; Murine experimental autoimmune encephalomyelitis; T cell migration; Thy-1 antigen; Thy-1 antigen; Murine experimental autoimmune encephalomyelitis; Congenic mouse strains; Chronic relapsing experimental autoimmune encephalomyelitis; Adoptive transfer; T cell migration
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/0165-5728(93)90242-Q

For the purpose of tracking the migration of donor T cells in chronic relapsing EAE (CREAE), a Thy-1 congenic mouse strain on the SJL genetic background was generated. T cells from these SJL. Thy-1 a mice (Thy-1.1 +) could be distinguished from those of SJL (Thy-1.2 +) by staining with specific anti-Thy-1 antibodies. In adoptive EAE, SJL.Thy-1 a mice exhibited similar chronic remitting and relapsing cycles as in SJL mice. Reciprocal transfer of adoptive EAE had also been successfully carried out between the two Thy-1 disparate strains. The lack of alloreactivity against the Thy-1 antigen was further demonstrated by the absence of reactivity in in vitro mixed lymphocyte cultures and in vivo skin graft rejection between the two strains. The establishment of the SJL.Thy-1 a mouse strain should prove to be a valuable tool for further dissecting the complex process of relapsing EAE.