Formation of nitric oxide hemoglobin in erythrocytes co-cultured with alveolar macrophages taken from bleomycin treated rats

• Published in: Biochemical and biophysical research communications Vol. 182; no. 1; pp. 151 - 157
• Main Authors: Huot, A.E., Kruszyna, H., Kruszyna, R., Smith, R.P., Hacker, M.P.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.01.1992; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Arginine - analogs & derivatives; Arginine - pharmacology; Biological and medical sciences; Bleomycin - pharmacology; Cell Communication; Cells, Cultured; Erythrocytes - cytology; Erythrocytes - physiology; Fundamental and applied biological sciences. Psychology; Hemoglobins - metabolism; Hemoproteins; Inflammation; Leukemia L1210 - pathology; Macrophages, Alveolar - cytology; Macrophages, Alveolar - drug effects; Macrophages, Alveolar - physiology; Male; Metalloproteins; Mice; Nitric Oxide - metabolism; Nitrites - metabolism; omega-N-Methylarginine; Proteins; Rats; Rats, Inbred Strains; Spectrophotometry; reactive nitrogen intermediates; bleomycin; nitric oxide; endothelium derived relaxing factor; intratracheal; AM taken from BLM treated animals; AM; minimal essential medium; bronchoalveolar lavage fluid cells; erythrocytes; donor horse serum; phosphate buffered saline; electron paramagnetic resonance spectroscopy; standard error of the mean; N G-monomethyl-arginine; Hemoprotein; Rat; Leukemia; Rodentia; Red blood cell; Absorption spectrometry; Vertebrata; Mammalia; Cell line; Mouse; Nitrogen monoxide; Hemoglobin; Endothelium derived relaxing factor; Release; Macrophage
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(05)80124-X

Alveolar macrophages, taken from rats treated with a single intratracheal dose of bleomycin, release reactive nitrogen intermediates in the form of nitric oxide which are cytostatic to murine leukemia L1210 cells. When cultured in the presence of erythrocytes the cytostatic activity of alveolar macrophages was inhibited which corresponded with an increase in nitrosylated hemoglobin content when compared with erythrocytes cultured alone. These results suggest that erythrocytes inhibit alveolar macrophage cytostatic activity by preventing reactive nitrogen intermediates from reaching target cells because the hemoglobin serves as a sink for reactive nitrogen intermediates in the form of nitric oxide.