Expression of the B cell-associated tyrosine kinase gene Lyn in primary neuroblastoma tumours and its modulation during the differentiation of neuroblastoma cell lines

• Published in: Biochemical and biophysical research communications Vol. 186; no. 3; pp. 1403 - 1409
• Main Authors: Bielke, Wolfgang, Ziemieki, Andrew, Kappos, Ludwig, Miescher, Guido C.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 14.08.1992; Elsevier
• Subjects: Amino Acid Sequence; B-Lymphocytes - enzymology; Base Sequence; Biological and medical sciences; Cell Differentiation; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Clone Cells; Drug Resistance - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Humans; Molecular and cellular biology; Molecular Sequence Data; Neuroblastoma; Oligodeoxyribonucleotides; Protein-Tyrosine Kinases - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Neoplasm - genetics; RNA, Neoplasm - isolation & purification; Sequence Homology, Nucleic Acid; Signal Transduction; src-Family Kinases; Transcription, Genetic; Tumor Cells, Cultured; Cell line; Enzyme; Neuroblastoma; Cell differentiation; Gene expression; Protein-tyrosine kinase; Retinoic acid
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(05)81562-1

The src-related intracellular protein tyrosine kinase Lyn is a signal transducing molecule for surface immunoglobulin M and is expressed predominantly in hemopoietic cells. We report here the expression of the lyn gene in human neuroblastoma. In surgical tumour samples lyn transcripts were found preferentially at early stages whereas they were barely detectable in highly malignant tumours. In a cloned human neuroblastoma cell line, Be(2)C, lyn mRNA levels increased during neuronal differentiation induced by retinoic acid. Lyn mRNA levels were undetectable and did not respond to retinoic acid in a glial-type neuroblastoma clone, SH-EP. Retinoic acid-induced glial differentiation was associated with a reduction of lyn transcripts in a clonal I-type neuroblastoma cell line, SH-IN, which shares properties of both neuronal- and glial-type clones. Like pp60 c-src Lyn may be involved in a signalling pathway of neuroblasts committed to neuronal differentiation.