Precise in situ localization of NCAM, ETS1, and D11S29 on human meiotic chromosomes

In order to sublocalize NCAM, ETS1, and the anonymous DNA fragment D11S29 within 11q23, in situ hybridization was performed on pachytene bivalents. Analysis of the grain distribution within the band 11q23 indicated that the chromosomal sublocalization of both NCAM and D11S29 was in 11q23.1, whereas...

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Published inCytogenetics and cell genetics Vol. 52; no. 1-2; p. 7
Main Authors Bello, M J, Salagnon, N, Rey, J A, Guichaoua, M R, Bergé-Lefranc, J L, Jordan, B R, Luciani, J M
Format Journal Article
LanguageEnglish
Published Switzerland 1989
Subjects
Chromosome Mapping
Chromosomes, Human, Pair 11
DNA - genetics
DNA Probes
Glycoproteins - genetics
Humans
Male
Meiosis
Membrane Proteins - genetics
Nucleic Acid Hybridization
Proto-Oncogenes - genetics
Testis - ultrastructure
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Summary:In order to sublocalize NCAM, ETS1, and the anonymous DNA fragment D11S29 within 11q23, in situ hybridization was performed on pachytene bivalents. Analysis of the grain distribution within the band 11q23 indicated that the chromosomal sublocalization of both NCAM and D11S29 was in 11q23.1, whereas ETS1 was found to be localized in 11q23.3. These results clearly demonstrate the usefulness of in situ hybridization applied to pachytene bivalents to obtain accurate gene sublocalization.
ISSN:0301-0171
DOI:10.1159/000132828