Composition of photosynthetic pigments in thylakoid membrane vesicles from spinach

Thylakoid membranes from spinach were fragmented mechanically and separated into vesicles originating from grana and stroma-exposed lamellae (Andreasson et al. (1988) Biochim Biophys Acta 936: 339-350). The grana vesicles were further fragmented and separated into smaller vesicles originating from d...

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Bibliographic Details
Published inPhotosynthesis research Vol. 35; no. 2; p. 171
Main Authors Juhler, R.K. (Odense Univ. (Denmark). Inst. of Biochemistry), Andreasson, E, Yu, S.G, Albertsson, P.A
Format Journal Article
LanguageEnglish
Published Netherlands 01.02.1993
Subjects
CAROTENOIDE
CAROTENOIDES
CAROTENOIDS
CHLOROPHYLLE
CHLOROPHYLLS
CHROMATOGRAPHIE LIQUIDE HAUTE PRESS
CLOROFILAS
CROMATOGRAFIA LIQUIDA ALTA PRESION
FOTOSINTESIS
HIGH PRESSURE LIQUID CHROMATOGRAPHY
MEMBRANA
MEMBRANE
MEMBRANES
PHOTOSYNTHESE
PHOTOSYNTHESIS
SPINACIA
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Summary:Thylakoid membranes from spinach were fragmented mechanically and separated into vesicles originating from grana and stroma-exposed lamellae (Andreasson et al. (1988) Biochim Biophys Acta 936: 339-350). The grana vesicles were further fragmented and separated into smaller vesicles originating from different parts of the grana (Svensson and Albertsson (1989) Photosynth Res 20: 249-259). All vesicles so obtained were analyzed with respect to chlorophyll and carotenoid composition by reverse phase HPLC. For all fractions the following relations (mole/mole) were found: 1 carotenoid per 4 chlorophyll (a+b), 2 lutein per 5 chlorophyll b and 5 violaxanthin per 100 chlorophyll (a + b). The contents of lutein and neoxanthin were each linearly related to chlorophyll b and β-carotene was linearly related to chlorophyll a.
Bibliography:F61
9301241
ISSN:0166-8595
1573-5079
DOI:10.1007/BF00014747